Ispinesib Mesylate-Induced Oxidative Stress Via Mir-30e-5p/BCL2L11 Axis in Acute Myocardial Infarction: A Comprehensive Bioinformatics And Experimental Validation

Abstract

Objective: In this study, we used molecular biology, cell biology and other techniques to reveal the relationship between miR-30e-5p and hypoxia-induced CA16 apoptosis at the molecular and cellular levels, further improve the molecular mechanism of acute myocardial infarction, and verify the target role of BCL2L11 in acute myocardial infarction through bioinformatics, so as to analyze and predict the therapeutic drugs for acute myocardial infarction, and provide early diagnosis and treatment targets for the prevention and treatment of acute myocardial infarction in clinical practice.

Methods: In this study, the datasets in the Gene Expression Comprehensive (GEO) database were used to conduct in-depth analysis of key genes by various methods, such as differential analysis, Wien analysis and weighted correlation network analysis (WGCNA). Subsequently, the correlation between the correlation factors and key genes was further analyzed. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) and lentiviral transfection experiments were carried out, miRNA-mRNA networks were constructed based on miRBase databases, three-dimensional structures were predicted with the help of RNAfold and Vfold3D databases, and drug sensitivity analysis was performed using RNAactDrug databases.

Results: Through classification, WGCNA clustering and Wien screening analysis, we successfully identified two differentially expressed genes closely related to apoptosis: PTEN and BCL2L11. The results of real-time quantitative polymerase chain reaction (RT-qPCR) and lentiviral infection experiments verified that the expression of BCL2L11 was consistent with the results of previous analysis. Finally, through miRNA-mRNA network and drug susceptibility analysis, we found that Ispinesib Mesylate, Bleomycin (50 uM)/miR-141-3p/BCL2L11 axis may be effective strategies for the treatment or prevention of acute myocardial infarction.

Conclusion: In this study, the key genes were analyzed and verified by cell culture and apoptosis assays. Subsequently, through a database of multiple drug targets, the concepts of Ispinesib Mesylate and Bleomycin (50 uM) / miR-141-3p / BCL2L11 axis were proposed for the first time, and two drugs of Ispinesib Mesylate and Bleomycin (50 uM) were predicted, and their 2D and 3D molecular structures were drawn, in order to provide a new perspective for the treatment and prevention of acute myocardial infarction.